p prkaa1 Search Results


p prkaa1  (Boster Bio)
94
Boster Bio p prkaa1
Fig. 5. NaF exposure induces elevated phos phorylation of <t>PRKAA1</t> expression in vivo and in vitro. (A) The hierarchical cluster heatmap of phosphorylated proteins in rat hippocampus. (B) Statistics of different phosphorylation sites and proteins in rat hippocampus. (C) Volcano plot of differentially phosphorylated proteins in rat hippocampus. The criteria for significance were set at logarithmic fold change (log2 FC) of > 1.2 or < 0.8 (P < 0.05). (D) Phosphorylation of PRKAA1 protein in rat hippocampus. (E-F) Representative western blot and relative quan tifications of PRKAA1 and p-PRKAA1 in rat hippocampus. (G-H) Representative western blot and relative quantifications of PRKAA1 and p-PRKAA1 in SH-SY5Y cells. All experiments were performed independently and repeated 3 times. The data were presented as the means ± SD. *P < 0.05 compare with the control group.
P Prkaa1, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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phospho ampk1  (Boster Bio)
93
Boster Bio phospho ampk1
Fig. 5. NaF exposure induces elevated phos phorylation of <t>PRKAA1</t> expression in vivo and in vitro. (A) The hierarchical cluster heatmap of phosphorylated proteins in rat hippocampus. (B) Statistics of different phosphorylation sites and proteins in rat hippocampus. (C) Volcano plot of differentially phosphorylated proteins in rat hippocampus. The criteria for significance were set at logarithmic fold change (log2 FC) of > 1.2 or < 0.8 (P < 0.05). (D) Phosphorylation of PRKAA1 protein in rat hippocampus. (E-F) Representative western blot and relative quan tifications of PRKAA1 and p-PRKAA1 in rat hippocampus. (G-H) Representative western blot and relative quantifications of PRKAA1 and p-PRKAA1 in SH-SY5Y cells. All experiments were performed independently and repeated 3 times. The data were presented as the means ± SD. *P < 0.05 compare with the control group.
Phospho Ampk1, supplied by Boster Bio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti-p-ampk1/2α (thr172) pab  (Santa Cruz Biotechnology)
90
Santa Cruz Biotechnology anti-p-ampk1/2α (thr172) pab
Fig. 5. NaF exposure induces elevated phos phorylation of <t>PRKAA1</t> expression in vivo and in vitro. (A) The hierarchical cluster heatmap of phosphorylated proteins in rat hippocampus. (B) Statistics of different phosphorylation sites and proteins in rat hippocampus. (C) Volcano plot of differentially phosphorylated proteins in rat hippocampus. The criteria for significance were set at logarithmic fold change (log2 FC) of > 1.2 or < 0.8 (P < 0.05). (D) Phosphorylation of PRKAA1 protein in rat hippocampus. (E-F) Representative western blot and relative quan tifications of PRKAA1 and p-PRKAA1 in rat hippocampus. (G-H) Representative western blot and relative quantifications of PRKAA1 and p-PRKAA1 in SH-SY5Y cells. All experiments were performed independently and repeated 3 times. The data were presented as the means ± SD. *P < 0.05 compare with the control group.
Anti P Ampk1/2α (Thr172) Pab, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti-p-ampk1/2α (thr172) pab/product/Santa Cruz Biotechnology
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anti p prkaa1 2  (Cell Signaling Technology Inc)
99
Cell Signaling Technology Inc anti p prkaa1 2
Fig. 5. NaF exposure induces elevated phos phorylation of <t>PRKAA1</t> expression in vivo and in vitro. (A) The hierarchical cluster heatmap of phosphorylated proteins in rat hippocampus. (B) Statistics of different phosphorylation sites and proteins in rat hippocampus. (C) Volcano plot of differentially phosphorylated proteins in rat hippocampus. The criteria for significance were set at logarithmic fold change (log2 FC) of > 1.2 or < 0.8 (P < 0.05). (D) Phosphorylation of PRKAA1 protein in rat hippocampus. (E-F) Representative western blot and relative quan tifications of PRKAA1 and p-PRKAA1 in rat hippocampus. (G-H) Representative western blot and relative quantifications of PRKAA1 and p-PRKAA1 in SH-SY5Y cells. All experiments were performed independently and repeated 3 times. The data were presented as the means ± SD. *P < 0.05 compare with the control group.
Anti P Prkaa1 2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti p prkaa1 2/product/Cell Signaling Technology Inc
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p ampk  (Proteintech)
93
Proteintech p ampk
rhGH improved lipid metabolism in db/db mice. (a) BODIPY-stained (green fluorescence) and DAPI-stained (blue fluorescence) photomicrographs and PAS staining in the cardiac tissue. (b) Cardiac <t>p-AMPK,</t> CPT-1, PGC1-ɑ, PPAR- α , CD36, and SCD-1 levels were measured by western blotting. (c) Relative protein expression was quantified. (d) Representative immunohistochemistry for p-AMPK, CPT-1, PGC1-ɑ, PPAR- α , CD36, and SCD-1 in the cardiac tissues. (e) Positive expression was quantified. Scale bar: 100 μ m. The arrows indicate positively stained cells. n = 3 per group. ∗ P < 0.05 vs. db/db+rhGH group, ∗∗ P < 0.01 vs. db/db+rhGH group, & P < 0.05 vs. db/db group, and # P < 0.01 vs. db/db group.
P Ampk, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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p prkaa1 ampk  (Cell Signaling Technology Inc)
99
Cell Signaling Technology Inc p prkaa1 ampk
rhGH improved lipid metabolism in db/db mice. (a) BODIPY-stained (green fluorescence) and DAPI-stained (blue fluorescence) photomicrographs and PAS staining in the cardiac tissue. (b) Cardiac <t>p-AMPK,</t> CPT-1, PGC1-ɑ, PPAR- α , CD36, and SCD-1 levels were measured by western blotting. (c) Relative protein expression was quantified. (d) Representative immunohistochemistry for p-AMPK, CPT-1, PGC1-ɑ, PPAR- α , CD36, and SCD-1 in the cardiac tissues. (e) Positive expression was quantified. Scale bar: 100 μ m. The arrows indicate positively stained cells. n = 3 per group. ∗ P < 0.05 vs. db/db+rhGH group, ∗∗ P < 0.01 vs. db/db+rhGH group, & P < 0.05 vs. db/db group, and # P < 0.01 vs. db/db group.
P Prkaa1 Ampk, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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p prkaa1 prkaa2  (Cell Signaling Technology Inc)
97
Cell Signaling Technology Inc p prkaa1 prkaa2
rhGH improved lipid metabolism in db/db mice. (a) BODIPY-stained (green fluorescence) and DAPI-stained (blue fluorescence) photomicrographs and PAS staining in the cardiac tissue. (b) Cardiac <t>p-AMPK,</t> CPT-1, PGC1-ɑ, PPAR- α , CD36, and SCD-1 levels were measured by western blotting. (c) Relative protein expression was quantified. (d) Representative immunohistochemistry for p-AMPK, CPT-1, PGC1-ɑ, PPAR- α , CD36, and SCD-1 in the cardiac tissues. (e) Positive expression was quantified. Scale bar: 100 μ m. The arrows indicate positively stained cells. n = 3 per group. ∗ P < 0.05 vs. db/db+rhGH group, ∗∗ P < 0.01 vs. db/db+rhGH group, & P < 0.05 vs. db/db group, and # P < 0.01 vs. db/db group.
P Prkaa1 Prkaa2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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p-prkaa1 (ser486) antibodies  (Thermo Fisher)
90
Thermo Fisher p-prkaa1 (ser486) antibodies
rhGH improved lipid metabolism in db/db mice. (a) BODIPY-stained (green fluorescence) and DAPI-stained (blue fluorescence) photomicrographs and PAS staining in the cardiac tissue. (b) Cardiac <t>p-AMPK,</t> CPT-1, PGC1-ɑ, PPAR- α , CD36, and SCD-1 levels were measured by western blotting. (c) Relative protein expression was quantified. (d) Representative immunohistochemistry for p-AMPK, CPT-1, PGC1-ɑ, PPAR- α , CD36, and SCD-1 in the cardiac tissues. (e) Positive expression was quantified. Scale bar: 100 μ m. The arrows indicate positively stained cells. n = 3 per group. ∗ P < 0.05 vs. db/db+rhGH group, ∗∗ P < 0.01 vs. db/db+rhGH group, & P < 0.05 vs. db/db group, and # P < 0.01 vs. db/db group.
P Prkaa1 (Ser486) Antibodies, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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primary antibodies  (Proteintech)
96
Proteintech primary antibodies
rhGH improved lipid metabolism in db/db mice. (a) BODIPY-stained (green fluorescence) and DAPI-stained (blue fluorescence) photomicrographs and PAS staining in the cardiac tissue. (b) Cardiac <t>p-AMPK,</t> CPT-1, PGC1-ɑ, PPAR- α , CD36, and SCD-1 levels were measured by western blotting. (c) Relative protein expression was quantified. (d) Representative immunohistochemistry for p-AMPK, CPT-1, PGC1-ɑ, PPAR- α , CD36, and SCD-1 in the cardiac tissues. (e) Positive expression was quantified. Scale bar: 100 μ m. The arrows indicate positively stained cells. n = 3 per group. ∗ P < 0.05 vs. db/db+rhGH group, ∗∗ P < 0.01 vs. db/db+rhGH group, & P < 0.05 vs. db/db group, and # P < 0.01 vs. db/db group.
Primary Antibodies, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti-p-prkaa1/2 (t172) (2535)  (Cell Signaling Technology Inc)
90
Cell Signaling Technology Inc anti-p-prkaa1/2 (t172) (2535)
rhGH improved lipid metabolism in db/db mice. (a) BODIPY-stained (green fluorescence) and DAPI-stained (blue fluorescence) photomicrographs and PAS staining in the cardiac tissue. (b) Cardiac <t>p-AMPK,</t> CPT-1, PGC1-ɑ, PPAR- α , CD36, and SCD-1 levels were measured by western blotting. (c) Relative protein expression was quantified. (d) Representative immunohistochemistry for p-AMPK, CPT-1, PGC1-ɑ, PPAR- α , CD36, and SCD-1 in the cardiac tissues. (e) Positive expression was quantified. Scale bar: 100 μ m. The arrows indicate positively stained cells. n = 3 per group. ∗ P < 0.05 vs. db/db+rhGH group, ∗∗ P < 0.01 vs. db/db+rhGH group, & P < 0.05 vs. db/db group, and # P < 0.01 vs. db/db group.
Anti P Prkaa1/2 (T172) (2535), supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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p ampk  (Boster Bio)
94
Boster Bio p ampk
rhGH improved lipid metabolism in db/db mice. (a) BODIPY-stained (green fluorescence) and DAPI-stained (blue fluorescence) photomicrographs and PAS staining in the cardiac tissue. (b) Cardiac <t>p-AMPK,</t> CPT-1, PGC1-ɑ, PPAR- α , CD36, and SCD-1 levels were measured by western blotting. (c) Relative protein expression was quantified. (d) Representative immunohistochemistry for p-AMPK, CPT-1, PGC1-ɑ, PPAR- α , CD36, and SCD-1 in the cardiac tissues. (e) Positive expression was quantified. Scale bar: 100 μ m. The arrows indicate positively stained cells. n = 3 per group. ∗ P < 0.05 vs. db/db+rhGH group, ∗∗ P < 0.01 vs. db/db+rhGH group, & P < 0.05 vs. db/db group, and # P < 0.01 vs. db/db group.
P Ampk, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/p ampk/product/Boster Bio
Average 94 stars, based on 1 article reviews
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Image Search Results


Fig. 5. NaF exposure induces elevated phos phorylation of PRKAA1 expression in vivo and in vitro. (A) The hierarchical cluster heatmap of phosphorylated proteins in rat hippocampus. (B) Statistics of different phosphorylation sites and proteins in rat hippocampus. (C) Volcano plot of differentially phosphorylated proteins in rat hippocampus. The criteria for significance were set at logarithmic fold change (log2 FC) of > 1.2 or < 0.8 (P < 0.05). (D) Phosphorylation of PRKAA1 protein in rat hippocampus. (E-F) Representative western blot and relative quan tifications of PRKAA1 and p-PRKAA1 in rat hippocampus. (G-H) Representative western blot and relative quantifications of PRKAA1 and p-PRKAA1 in SH-SY5Y cells. All experiments were performed independently and repeated 3 times. The data were presented as the means ± SD. *P < 0.05 compare with the control group.

Journal: Ecotoxicology and environmental safety

Article Title: PRKAA1 induces aberrant mitophagy in a PINK1/Parkin-dependent manner, contributing to fluoride-induced developmental neurotoxicity.

doi: 10.1016/j.ecoenv.2023.114772

Figure Lengend Snippet: Fig. 5. NaF exposure induces elevated phos phorylation of PRKAA1 expression in vivo and in vitro. (A) The hierarchical cluster heatmap of phosphorylated proteins in rat hippocampus. (B) Statistics of different phosphorylation sites and proteins in rat hippocampus. (C) Volcano plot of differentially phosphorylated proteins in rat hippocampus. The criteria for significance were set at logarithmic fold change (log2 FC) of > 1.2 or < 0.8 (P < 0.05). (D) Phosphorylation of PRKAA1 protein in rat hippocampus. (E-F) Representative western blot and relative quan tifications of PRKAA1 and p-PRKAA1 in rat hippocampus. (G-H) Representative western blot and relative quantifications of PRKAA1 and p-PRKAA1 in SH-SY5Y cells. All experiments were performed independently and repeated 3 times. The data were presented as the means ± SD. *P < 0.05 compare with the control group.

Article Snippet: PRKAA1, p-PRKAA1 (Thr172), Parkin, and Cyt C antibodies were obtained from Boster Biotech (China).

Techniques: Expressing, In Vivo, In Vitro, Phospho-proteomics, Western Blot, Control

Fig. 6. DM inhibits aberrant mitophagy by pre venting the phosphorylation of PRKAA1 in vitro. (A-B) Representative western blot and relative quantifications of PRKAA1and p-PRKAA1 in SH- SY5Y cells. (C-D) Representative western blot and relative quantifications of PINK1 and Parkin in SH-SY5Y cells. (E-F) Representative images and relative quantifications of immunofluores cence staining of PINK1 and Mito-tracker, after DM intervention. The scale bar represents 50 µm. (G-H) Representative western blot and relative quantifications of TOMM-20 in SH-SY5Y cells. All experiments were performed independently and repeated 3 times. The data were presented as the means ± SD. *P < 0.05 compare with the control group, @P < 0.05 compare with the NaF group.

Journal: Ecotoxicology and environmental safety

Article Title: PRKAA1 induces aberrant mitophagy in a PINK1/Parkin-dependent manner, contributing to fluoride-induced developmental neurotoxicity.

doi: 10.1016/j.ecoenv.2023.114772

Figure Lengend Snippet: Fig. 6. DM inhibits aberrant mitophagy by pre venting the phosphorylation of PRKAA1 in vitro. (A-B) Representative western blot and relative quantifications of PRKAA1and p-PRKAA1 in SH- SY5Y cells. (C-D) Representative western blot and relative quantifications of PINK1 and Parkin in SH-SY5Y cells. (E-F) Representative images and relative quantifications of immunofluores cence staining of PINK1 and Mito-tracker, after DM intervention. The scale bar represents 50 µm. (G-H) Representative western blot and relative quantifications of TOMM-20 in SH-SY5Y cells. All experiments were performed independently and repeated 3 times. The data were presented as the means ± SD. *P < 0.05 compare with the control group, @P < 0.05 compare with the NaF group.

Article Snippet: PRKAA1, p-PRKAA1 (Thr172), Parkin, and Cyt C antibodies were obtained from Boster Biotech (China).

Techniques: Phospho-proteomics, In Vitro, Western Blot, Staining, Control

rhGH improved lipid metabolism in db/db mice. (a) BODIPY-stained (green fluorescence) and DAPI-stained (blue fluorescence) photomicrographs and PAS staining in the cardiac tissue. (b) Cardiac p-AMPK, CPT-1, PGC1-ɑ, PPAR- α , CD36, and SCD-1 levels were measured by western blotting. (c) Relative protein expression was quantified. (d) Representative immunohistochemistry for p-AMPK, CPT-1, PGC1-ɑ, PPAR- α , CD36, and SCD-1 in the cardiac tissues. (e) Positive expression was quantified. Scale bar: 100 μ m. The arrows indicate positively stained cells. n = 3 per group. ∗ P < 0.05 vs. db/db+rhGH group, ∗∗ P < 0.01 vs. db/db+rhGH group, & P < 0.05 vs. db/db group, and # P < 0.01 vs. db/db group.

Journal: Oxidative Medicine and Cellular Longevity

Article Title: Recombinant Human Growth Hormone Inhibits Lipotoxicity, Oxidative Stress, and Apoptosis in a Mouse Model of Diabetic Cardiomyopathy

doi: 10.1155/2021/3899356

Figure Lengend Snippet: rhGH improved lipid metabolism in db/db mice. (a) BODIPY-stained (green fluorescence) and DAPI-stained (blue fluorescence) photomicrographs and PAS staining in the cardiac tissue. (b) Cardiac p-AMPK, CPT-1, PGC1-ɑ, PPAR- α , CD36, and SCD-1 levels were measured by western blotting. (c) Relative protein expression was quantified. (d) Representative immunohistochemistry for p-AMPK, CPT-1, PGC1-ɑ, PPAR- α , CD36, and SCD-1 in the cardiac tissues. (e) Positive expression was quantified. Scale bar: 100 μ m. The arrows indicate positively stained cells. n = 3 per group. ∗ P < 0.05 vs. db/db+rhGH group, ∗∗ P < 0.01 vs. db/db+rhGH group, & P < 0.05 vs. db/db group, and # P < 0.01 vs. db/db group.

Article Snippet: The primary antibodies were against ANP (rabbit anti-ANP antibody, 1 : 500; Invitrogen, USA), BNP (rabbit anti-BNP antibody, 1 : 500; Invitrogen), CD36 (rabbit anti-CD36 antibody, 1 : 1000; Proteintech), SCD-1 (rabbit anti-SCD-1 antibody, 1 : 1000; Abcam), PGC1-ɑ (rabbit anti-PGC1-ɑ antibody, 1 : 1000; Proteintech), CPT-1 (rabbit anti-CPT-1 antibody, 1 : 1000; Proteintech), p-AMPK (rabbit anti-p-AMPK antibody, 1 : 1000; Proteintech), NRF2 (rabbit anti-NRF2 antibody, 1 : 500; Proteintech), HO-1 (rabbit anti-HO-1 antibody, 1 : 1000; Abcam), PPAR- α (rabbit anti-PPAR- α antibody, 1 : 500; Proteintech), SOD (rabbit anti-SOD antibody, 1 : 2000; Proteintech), NOX4 (rabbit anti-NOX4 antibody, 1 : 1000; Proteintech), CAT (rabbit anti-CAT antibody, 1 : 1000; Proteintech), BAX (rabbit anti-BAX antibody, 1 : 5000; Proteintech), Bcl-2 (rabbit anti-Bcl-2 antibody, 1 : 2000; Proteintech), cleaved caspase-3 (rabbit anti-cleaved caspase-3 antibody, 1 : 1000; Cell Signaling Technology), t-caspase-3 (rabbit anti-t-caspase-3 antibody, 1 : 1000; Proteintech), and anti- β -actin (1 : 1000; Proteintech).

Techniques: Staining, Fluorescence, Western Blot, Expressing, Immunohistochemistry